The monkeypox virus is a serious threat to public health, and early detection is essential for preventing its spread. Rapid diagnosis are an important tool for identifying infected individuals. Metagenomics sequencing offers a more affordable and accessible option for detecting monkeypox infections. By sequencing the genomes of viruses present in a sample, metagenomics sequencing can provide information about the identity and abundance of viruses in a population. This information can be used to track the spread of monkeypox and identify potential hot spots for transmission. In addition, metagenomics sequencing can reveal changes in the viral microbiome that may be associated with disease progression. This information can help researchers develop more effective treatments for monkeypox.